human dermal fibroblast hdf hs68 cells Search Results


human foreskin fibroblasts  (ATCC)
96
ATCC human foreskin fibroblasts
Human Foreskin Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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normal human foreskin fibroblast cell line hs68  (JCRB Cell Bank)
90
JCRB Cell Bank normal human foreskin fibroblast cell line hs68
A. The yeast two-hybrid analysis was conducted using pPC86 (AD)/full-length human SGTA (derived from a normal heart cDNA library) and pDBLeu (BD)/full-length human REIC/DKK-3 plasmids. The blue colonies indicate those with an interaction between the two proteins. B. For the pull-down (PD) assay, the full-length cDNA of human REIC/DKK-3 and SGTA was cloned into the pFN21A and pMACS Kk.HA-C plasmids, respectively. Cell lysates from Halo-tagged REIC/DKK-3- and/or HA-tagged SGTA-transfected 293T cells were analyzed. The sample pulled down using Halo-tagged REIC/DKK-3 was analyzed by Western blotting (WB) using anti-HA antibody. C. REIC/DKK-3 and SGTA protein expression in 293T, PC3 and <t>Hs68</t> cells was analyzed by Western blotting. Coomassie Brilliant Blue (CBB) staining of the membrane is shown as a loading control. D. The co-localization of REIC/DKK-3 and SGTA was examined by double immunofluorescence staining and observed by fluorescence microscopy. The images in green and red show the intracellular localization of REIC/DKK-3 and SGTA, respectively. The areas of overlap between REIC/DKK-3 and SGTA are shown in yellow in the merged image.
Normal Human Foreskin Fibroblast Cell Line Hs68, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hs68 atcc crl 1635 rrid cvcl 0839 human  (ATCC)
99
ATCC hs68 atcc crl 1635 rrid cvcl 0839 human
A. The yeast two-hybrid analysis was conducted using pPC86 (AD)/full-length human SGTA (derived from a normal heart cDNA library) and pDBLeu (BD)/full-length human REIC/DKK-3 plasmids. The blue colonies indicate those with an interaction between the two proteins. B. For the pull-down (PD) assay, the full-length cDNA of human REIC/DKK-3 and SGTA was cloned into the pFN21A and pMACS Kk.HA-C plasmids, respectively. Cell lysates from Halo-tagged REIC/DKK-3- and/or HA-tagged SGTA-transfected 293T cells were analyzed. The sample pulled down using Halo-tagged REIC/DKK-3 was analyzed by Western blotting (WB) using anti-HA antibody. C. REIC/DKK-3 and SGTA protein expression in 293T, PC3 and <t>Hs68</t> cells was analyzed by Western blotting. Coomassie Brilliant Blue (CBB) staining of the membrane is shown as a loading control. D. The co-localization of REIC/DKK-3 and SGTA was examined by double immunofluorescence staining and observed by fluorescence microscopy. The images in green and red show the intracellular localization of REIC/DKK-3 and SGTA, respectively. The areas of overlap between REIC/DKK-3 and SGTA are shown in yellow in the merged image.
Hs68 Atcc Crl 1635 Rrid Cvcl 0839 Human, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human fibroblasts hs68  (BioResource International Inc)
90
BioResource International Inc human fibroblasts hs68
A. The yeast two-hybrid analysis was conducted using pPC86 (AD)/full-length human SGTA (derived from a normal heart cDNA library) and pDBLeu (BD)/full-length human REIC/DKK-3 plasmids. The blue colonies indicate those with an interaction between the two proteins. B. For the pull-down (PD) assay, the full-length cDNA of human REIC/DKK-3 and SGTA was cloned into the pFN21A and pMACS Kk.HA-C plasmids, respectively. Cell lysates from Halo-tagged REIC/DKK-3- and/or HA-tagged SGTA-transfected 293T cells were analyzed. The sample pulled down using Halo-tagged REIC/DKK-3 was analyzed by Western blotting (WB) using anti-HA antibody. C. REIC/DKK-3 and SGTA protein expression in 293T, PC3 and <t>Hs68</t> cells was analyzed by Western blotting. Coomassie Brilliant Blue (CBB) staining of the membrane is shown as a loading control. D. The co-localization of REIC/DKK-3 and SGTA was examined by double immunofluorescence staining and observed by fluorescence microscopy. The images in green and red show the intracellular localization of REIC/DKK-3 and SGTA, respectively. The areas of overlap between REIC/DKK-3 and SGTA are shown in yellow in the merged image.
Human Fibroblasts Hs68, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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materials human foreskin fibroblasts  (ATCC)
97
ATCC materials human foreskin fibroblasts
A. The yeast two-hybrid analysis was conducted using pPC86 (AD)/full-length human SGTA (derived from a normal heart cDNA library) and pDBLeu (BD)/full-length human REIC/DKK-3 plasmids. The blue colonies indicate those with an interaction between the two proteins. B. For the pull-down (PD) assay, the full-length cDNA of human REIC/DKK-3 and SGTA was cloned into the pFN21A and pMACS Kk.HA-C plasmids, respectively. Cell lysates from Halo-tagged REIC/DKK-3- and/or HA-tagged SGTA-transfected 293T cells were analyzed. The sample pulled down using Halo-tagged REIC/DKK-3 was analyzed by Western blotting (WB) using anti-HA antibody. C. REIC/DKK-3 and SGTA protein expression in 293T, PC3 and <t>Hs68</t> cells was analyzed by Western blotting. Coomassie Brilliant Blue (CBB) staining of the membrane is shown as a loading control. D. The co-localization of REIC/DKK-3 and SGTA was examined by double immunofluorescence staining and observed by fluorescence microscopy. The images in green and red show the intracellular localization of REIC/DKK-3 and SGTA, respectively. The areas of overlap between REIC/DKK-3 and SGTA are shown in yellow in the merged image.
Materials Human Foreskin Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hs68  (ATCC)
96
ATCC hs68
(A) Cells were treated with ciclopirox for 96 hours. Curves showing that ciclopirox suppresses viability of neuroblastoma cells but not <t>HS68</t> cells, a normal human fibroblast cell line. The IC50 of CPX to each cell line was summarized in the table. The proliferation index for each cell line was determined and summarized in the table.
Hs68, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hs68 cells  (Blackwell Science Ltd)
90
Blackwell Science Ltd hs68 cells
(A) Cells were treated with ciclopirox for 96 hours. Curves showing that ciclopirox suppresses viability of neuroblastoma cells but not <t>HS68</t> cells, a normal human fibroblast cell line. The IC50 of CPX to each cell line was summarized in the table. The proliferation index for each cell line was determined and summarized in the table.
Hs68 Cells, supplied by Blackwell Science Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hs68 (ecacc n° 89051701  (Millipore)
90
Millipore hs68 (ecacc n° 89051701
(A) Cells were treated with ciclopirox for 96 hours. Curves showing that ciclopirox suppresses viability of neuroblastoma cells but not <t>HS68</t> cells, a normal human fibroblast cell line. The IC50 of CPX to each cell line was summarized in the table. The proliferation index for each cell line was determined and summarized in the table.
Hs68 (Ecacc N° 89051701, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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normal human fibroblasts hs68  (SACRI Antibody)
90
SACRI Antibody normal human fibroblasts hs68
(A) Cells were treated with ciclopirox for 96 hours. Curves showing that ciclopirox suppresses viability of neuroblastoma cells but not <t>HS68</t> cells, a normal human fibroblast cell line. The IC50 of CPX to each cell line was summarized in the table. The proliferation index for each cell line was determined and summarized in the table.
Normal Human Fibroblasts Hs68, supplied by SACRI Antibody, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary fibroblast cells hs68  (Santa Cruz Biotechnology)
91
Santa Cruz Biotechnology primary fibroblast cells hs68
(A) Cells were treated with ciclopirox for 96 hours. Curves showing that ciclopirox suppresses viability of neuroblastoma cells but not <t>HS68</t> cells, a normal human fibroblast cell line. The IC50 of CPX to each cell line was summarized in the table. The proliferation index for each cell line was determined and summarized in the table.
Primary Fibroblast Cells Hs68, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hs 68 cells (human foreskin fibroblasts)  (European Collection of Authenticated Cell Cultures)
90
European Collection of Authenticated Cell Cultures hs 68 cells (human foreskin fibroblasts)
(A) Cells were treated with ciclopirox for 96 hours. Curves showing that ciclopirox suppresses viability of neuroblastoma cells but not <t>HS68</t> cells, a normal human fibroblast cell line. The IC50 of CPX to each cell line was summarized in the table. The proliferation index for each cell line was determined and summarized in the table.
Hs 68 Cells (Human Foreskin Fibroblasts), supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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oligonucleotides cox1_f (qpcr primer)  (Eurofins)
90
Eurofins oligonucleotides cox1_f (qpcr primer)
(A) Cells were treated with ciclopirox for 96 hours. Curves showing that ciclopirox suppresses viability of neuroblastoma cells but not <t>HS68</t> cells, a normal human fibroblast cell line. The IC50 of CPX to each cell line was summarized in the table. The proliferation index for each cell line was determined and summarized in the table.
Oligonucleotides Cox1 F (Qpcr Primer), supplied by Eurofins, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A. The yeast two-hybrid analysis was conducted using pPC86 (AD)/full-length human SGTA (derived from a normal heart cDNA library) and pDBLeu (BD)/full-length human REIC/DKK-3 plasmids. The blue colonies indicate those with an interaction between the two proteins. B. For the pull-down (PD) assay, the full-length cDNA of human REIC/DKK-3 and SGTA was cloned into the pFN21A and pMACS Kk.HA-C plasmids, respectively. Cell lysates from Halo-tagged REIC/DKK-3- and/or HA-tagged SGTA-transfected 293T cells were analyzed. The sample pulled down using Halo-tagged REIC/DKK-3 was analyzed by Western blotting (WB) using anti-HA antibody. C. REIC/DKK-3 and SGTA protein expression in 293T, PC3 and Hs68 cells was analyzed by Western blotting. Coomassie Brilliant Blue (CBB) staining of the membrane is shown as a loading control. D. The co-localization of REIC/DKK-3 and SGTA was examined by double immunofluorescence staining and observed by fluorescence microscopy. The images in green and red show the intracellular localization of REIC/DKK-3 and SGTA, respectively. The areas of overlap between REIC/DKK-3 and SGTA are shown in yellow in the merged image.

Journal: Oncotarget

Article Title: Tumor suppressor REIC/DKK-3 and co-chaperone SGTA: Their interaction and roles in the androgen sensitivity

doi: 10.18632/oncotarget.6488

Figure Lengend Snippet: A. The yeast two-hybrid analysis was conducted using pPC86 (AD)/full-length human SGTA (derived from a normal heart cDNA library) and pDBLeu (BD)/full-length human REIC/DKK-3 plasmids. The blue colonies indicate those with an interaction between the two proteins. B. For the pull-down (PD) assay, the full-length cDNA of human REIC/DKK-3 and SGTA was cloned into the pFN21A and pMACS Kk.HA-C plasmids, respectively. Cell lysates from Halo-tagged REIC/DKK-3- and/or HA-tagged SGTA-transfected 293T cells were analyzed. The sample pulled down using Halo-tagged REIC/DKK-3 was analyzed by Western blotting (WB) using anti-HA antibody. C. REIC/DKK-3 and SGTA protein expression in 293T, PC3 and Hs68 cells was analyzed by Western blotting. Coomassie Brilliant Blue (CBB) staining of the membrane is shown as a loading control. D. The co-localization of REIC/DKK-3 and SGTA was examined by double immunofluorescence staining and observed by fluorescence microscopy. The images in green and red show the intracellular localization of REIC/DKK-3 and SGTA, respectively. The areas of overlap between REIC/DKK-3 and SGTA are shown in yellow in the merged image.

Article Snippet: The normal human foreskin fibroblast cell line Hs68 was provided by JCRB Cell Bank (Osaka, Japan).

Techniques: Derivative Assay, cDNA Library Assay, Clone Assay, Transfection, Western Blot, Expressing, Staining, Membrane, Control, Double Immunofluorescence Staining, Fluorescence, Microscopy

(A) Cells were treated with ciclopirox for 96 hours. Curves showing that ciclopirox suppresses viability of neuroblastoma cells but not HS68 cells, a normal human fibroblast cell line. The IC50 of CPX to each cell line was summarized in the table. The proliferation index for each cell line was determined and summarized in the table.

Journal: Cancer research

Article Title: Targeting histone demethylases in MYC-driven neuroblastomas with ciclopirox

doi: 10.1158/0008-5472.CAN-16-0826

Figure Lengend Snippet: (A) Cells were treated with ciclopirox for 96 hours. Curves showing that ciclopirox suppresses viability of neuroblastoma cells but not HS68 cells, a normal human fibroblast cell line. The IC50 of CPX to each cell line was summarized in the table. The proliferation index for each cell line was determined and summarized in the table.

Article Snippet: Human 293T, HS68, BE(2)-C, SK-N-AS, IMR-32, SK-N-SH, and U2OS cell lines were purchased from the American Type Culture Collection (ATCC) between 2012 to 2014.

Techniques: